M. lignano Single Cells Juveniles
Mlig455
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MligTC455_36944 (NR2F1)

Mlig455 TC Mlig455 genes Homologs Pfam Full annotation
MligTC455_36944 Mlig455_065298
Mlig455_065320		 NR2F1; Nr2f1; svp Ligand-binding domain of nuclear hormone receptor;
Zinc finger, C4 type (two domains)		 Mlig455_065298:
{TRANSCRIPTION_FACTOR}
{Pfam: Zinc finger, C4 type (two domains) [PF00105.20, score=100.7]; Ligand-binding domain of nuclear hormone receptor [PF00104.32, score=95.1]}
{Human: ENSG00000175745, NR2F1, nuclear receptor subfamily 2 group F member 1, [RH, Score=241, Expect=4e-73]; ENSG00000185551, NR2F2, nuclear receptor subfamily 2 group F member 2, [RH, Score=240, Expect=2e-72]}
{Mouse: ENSMUSG00000069171, Nr2f1, nuclear receptor subfamily 2, group F, member 1, [RH, Score=240, Expect=2e-72]; ENSMUSG00000030551, Nr2f2, nuclear receptor subfamily 2, group F, member 2, [RH, Score=240, Expect=1e-72]}
{Dmel: FBgn0003651, svp, seven up, [RH, Score=240, Expect=3e-71]}
{Celegans: WBGene00006790, unc-55, UNC-55b isoform, [RH, Score=150, Expect=4e-40]}
{Smed: dd_Smed_v6_33286_0_1, dd_Smed_v6_33286_0_1, [RH, Score=211, Expect=4e-61]}

Mlig455_065320:
{TRANSCRIPTION_FACTOR}
{Pfam: Ligand-binding domain of nuclear hormone receptor [PF00104.32, score=76.1]}
{Human: ENSG00000175745, NR2F1, nuclear receptor subfamily 2 group F member 1, [Score=215, Expect=6e-67]; ENSG00000185551, NR2F2, nuclear receptor subfamily 2 group F member 2, [Score=214, Expect=5e-66]}
{Mouse: ENSMUSG00000069171, Nr2f1, nuclear receptor subfamily 2, group F, member 1, [Score=215, Expect=2e-68]; ENSMUSG00000030551, Nr2f2, nuclear receptor subfamily 2, group F, member 2, [Score=214, Expect=4e-66]}
{Dmel: FBgn0003651, svp, seven up, [Score=211, Expect=2e-66]}
{Celegans: WBGene00006790, unc-55, UNC-55b isoform, [Score=69.3, Expect=4e-13]}
{Smed: dd_Smed_v6_33286_0_1, dd_Smed_v6_33286_0_1, [Score=183, Expect=9e-54]}

Links: Tomoseq.

Expression, Z-scaled

Variance, Z-scaled

Clusters

Expression, raw

Similar genes by expression

Nr. Mlig455 TC Pearson corr. Mlig455 genes Homologs Pfam Full annotation
1. MligTC455_23005 0.703 Mlig455_001754 Details
2. MligTC455_26383 0.703 Mlig455_001537
Mlig455_006135
Mlig455_009128
Mlig455_022786
Mlig455_026372
Mlig455_031457
Mlig455_056311
Mlig455_058067		 Transposase IS4 Details
3. MligTC455_32829 0.703 Mlig455_006704 ACOT1; Acot2 BAAT / Acyl-CoA thioester hydrolase C terminal Details
4. MligTC455_02752 0.703 Mlig455_006874 Details
5. MligTC455_04178 0.703 Mlig455_007825 PLSCR1; Plscr2; scramb1 Scramblase Details (TF)
6. MligTC455_38213 0.703 Mlig455_009922 Details
7. MligTC455_13773 0.703 Mlig455_010827 Details
8. MligTC455_14052 0.703 Mlig455_013563 Details
9. MligTC455_14053 0.703 Mlig455_013617 Details
10. MligTC455_38215 0.703 Mlig455_017163 Details
11. MligTC455_08514 0.703 Mlig455_025008 Reverse transcriptase (RNA-dependent DNA polymerase) Details
12. MligTC455_55739 0.703 Mlig455_027550 Integrase core domain Details
13. MligTC455_46625 0.703 Mlig455_031223 TTLL3A; TTLL8; Ttll8 Tubulin-tyrosine ligase family Details
14. MligTC455_39919 0.703 Mlig455_009244
Mlig455_009648
Mlig455_037054		 Details
15. MligTC455_42358 0.703 Mlig455_037708 Details
16. MligTC455_29689 0.703 Mlig455_038052 ANKRD50; Ankrd50; mask Ankyrin repeats (3 copies) Details
17. MligTC455_21028 0.703 Mlig455_040137 N; NOTCH2; Notch3 Calcium-binding EGF domain Details
18. MligTC455_03357 0.703 Mlig455_041258 2010300C02Rik; CCDC15; Muc68E Details
19. MligTC455_21370 0.703 Mlig455_016208
Mlig455_050599
Mlig455_062001
Mlig455_069812		 Details
20. MligTC455_34062 0.703 Mlig455_051516 SecA DEAD-like domain Details
21. MligTC455_03358 0.703 Mlig455_053079 Acan; Muc68E Methyl-accepting chemotaxis protein (MCP) signalling domain Details
22. MligTC455_38217 0.703 Mlig455_038564
Mlig455_052193
Mlig455_053460		 Details
23. MligTC455_24920 0.703 Mlig455_055084 Gm14025 Vinculin family Details
24. MligTC455_34920 0.703 Mlig455_059390
Mlig455_065559		 Details
25. MligTC455_52025 0.579 Mlig455_045543
Mlig455_052640
Mlig455_061469
Mlig455_065710		 DDE superfamily endonuclease Details
26. MligTC455_23006 0.522 Mlig455_001776 Details
27. MligTC455_46857 0.522 Mlig455_027226 TJP2; Tjp1; pyd PDZ domain Details
28. MligTC455_16340 0.496 Mlig455_013287
Mlig455_035931		 Details
29. MligTC455_27642 0.495 Mlig455_006426 NOTCH1; Notch1; crb EGF-like domain Details
30. MligTC455_32823 0.495 Mlig455_006535 ACOT6; Acot1 BAAT / Acyl-CoA thioester hydrolase C terminal Details
31. MligTC455_32825 0.495 Mlig455_006650 ACOT6; Acot2 BAAT / Acyl-CoA thioester hydrolase C terminal Details
32. MligTC455_32826 0.495 Mlig455_006701 ACOT6; Acot4 BAAT / Acyl-CoA thioester hydrolase C terminal Details
33. MligTC455_01281 0.495 Mlig455_024412 Details
34. MligTC455_01282 0.495 Mlig455_024527 Details
35. MligTC455_07164 0.495 Mlig455_037864 Ankyrin repeats (3 copies) Details
36. MligTC455_03037 0.495 Mlig455_058181
Mlig455_058205		 CG6406; FAM126A; Fam126b Hyccin Details
37. MligTC455_30451 0.495 Mlig455_016495
Mlig455_058254		 CG14207; HSPB1; Hspb1 Hsp20/alpha crystallin family Details
38. MligTC455_29782 0.495 Mlig455_061628 CG7509; PXDN; Pxdn Leucine rich repeat Details
39. MligTC455_26938 0.479 Mlig455_006444 Mppe Calcineurin-like phosphoesterase Details
40. MligTC455_33256 0.479 Mlig455_006849 MUC1 Lectin C-type domain Details
41. MligTC455_11231 0.479 Mlig455_015432 CG13229 Serpentine type 7TM GPCR chemoreceptor Srw Details
42. MligTC455_09518 0.479 Mlig455_016254 Details
43. MligTC455_45775 0.479 Mlig455_031567 NYNRIN; Nynrin Integrase zinc binding domain Details
44. MligTC455_55027 0.479 Mlig455_032148 CG13229 Serpentine type 7TM GPCR chemoreceptor Srw Details
45. MligTC455_29612 0.479 Mlig455_041751 Mppe Calcineurin-like phosphoesterase Details
46. MligTC455_12250 0.479 Mlig455_044202 Details
47. MligTC455_07423 0.479 Mlig455_054448 Details
48. MligTC455_55490 0.479 Mlig455_058627 Transposase Details
49. MligTC455_01166 0.479 Mlig455_058720 Drl-2; RYK; Ryk WIF domain Details
50. MligTC455_31958 0.479 Mlig455_023378
Mlig455_031273
Mlig455_064223		 PRR9 Helix-turn-helix domain Details
51. MligTC455_12901 0.479 Mlig455_067948 MULE transposase domain Details
52. MligTC455_10902 0.479 Mlig455_026575
Mlig455_026665
Mlig455_071099		 Details
53. MligTC455_30646 0.471 Mlig455_024994 SACS; Sacs Details
54. MligTC455_12073 0.46 Mlig455_060553 Details
55. MligTC455_32830 0.451 Mlig455_006541 ACOT1; Acot4 BAAT / Acyl-CoA thioester hydrolase C terminal Details
56. MligTC455_32119 0.451 Mlig455_039471 CG1885; UROS; Uros Uroporphyrinogen-III synthase HemD Details
57. MligTC455_36817 0.451 Mlig455_028298
Mlig455_063440
Mlig455_063473		 ANK3; Ankrd44; HACE1; nompC Ankyrin repeats (3 copies) Details
58. MligTC455_47886 0.449 Mlig455_052268
Mlig455_052359		 Details
59. MligTC455_54649 0.448 Mlig455_046551 Details
60. MligTC455_37663 0.439 Mlig455_023989
Mlig455_023996
Mlig455_024059		 MAN1B1; MAN1C1; Man1c1; alpha-Man-Ic Glycosyl hydrolase family 47 Details
61. MligTC455_17766 0.434 Mlig455_053411 SYTL4; Sytl5; btsz C2 domain Details
62. MligTC455_42167 0.427 Mlig455_046550 Details
63. MligTC455_32828 0.417 Mlig455_006652 ACOT1; Acot4 BAAT / Acyl-CoA thioester hydrolase C terminal Details
64. MligTC455_40705 0.417 Mlig455_052679 CCDC174 Domain of unknown function (DUF4078) Details
65. MligTC455_33562 0.403 Mlig455_012489 Details
66. MligTC455_05198 0.403 Mlig455_045613 Domain of unknown function (DUF4081) Details
67. MligTC455_07616 0.403 Mlig455_065059 Details
68. MligTC455_34539 0.403 Mlig455_065631 Immunoglobulin domain Details
69. MligTC455_29243 0.403 Mlig455_007367
Mlig455_066683		 Details
70. MligTC455_04469 0.393 Mlig455_045497 Amt Ammonium Transporter Family Details
71. MligTC455_22441 0.393 Mlig455_046125
Mlig455_051765		 Details
72. MligTC455_33131 0.392 Mlig455_022594
Mlig455_026005
Mlig455_070429		 Hil; KY; Ky Helix-turn-helix domain of transposase family ISL3;
Transglutaminase-like superfamily		 Details
73. MligTC455_35037 0.377 Mlig455_052487 CG8195; SLC35F5; Slc35f5 Details
74. MligTC455_28685 0.377 Mlig455_052645 Integrase core domain Details
75. MligTC455_42166 0.374 Mlig455_001548 Details
76. MligTC455_09350 0.373 Mlig455_034571 Details
77. MligTC455_15888 0.373 Mlig455_056804
Mlig455_056842		 Details
78. MligTC455_42019 0.37 Mlig455_014008 CEP57; Cep57 Centrosome localisation domain of Cep57 Details
79. MligTC455_49314 0.362 Mlig455_001336 CG1090; SLC24A3; Slc24a3 Sodium/calcium exchanger protein Details
80. MligTC455_06407 0.362 Mlig455_055014 Details
81. MligTC455_31288 0.36 Mlig455_056331 BEN domain Details
82. MligTC455_35034 0.359 Mlig455_012278 CG8195; SLC35F5; Slc35f5 Details
83. MligTC455_47962 0.359 Mlig455_013564
Mlig455_013618		 Details
84. MligTC455_03494 0.354 Mlig455_049159
Mlig455_049213		 LBX1; Lbx1; lbe; lbl Homeodomain Details (TF)
85. MligTC455_49315 0.354 Mlig455_070598 CG1090; SLC24A3; Slc24a3 Sodium/calcium exchanger protein Details
86. MligTC455_29906 0.353 Mlig455_023040
Mlig455_069770		 MAP3K4; Map3k4; Mekk1 Protein kinase domain Details
87. MligTC455_23100 0.35 Mlig455_019660 Details
88. MligTC455_23101 0.348 Mlig455_048080 Details
89. MligTC455_20469 0.347 Mlig455_002370 CYP2C8; Cyp18a1; Cyp2b19 Cytochrome P450 Details
90. MligTC455_28542 0.347 Mlig455_002557 CG5987; TTLL6; Ttll13 Tubulin-tyrosine ligase family Details
91. MligTC455_18643 0.347 Mlig455_002712 MFSD13A; Mfsd13a MFS/sugar transport protein Details
92. MligTC455_52442 0.347 Mlig455_007385 Details
93. MligTC455_00854 0.347 Mlig455_022587 Reverse transcriptase (RNA-dependent DNA polymerase) Details
94. MligTC455_02589 0.347 Mlig455_027271 MX1; Mx2 Dynamin central region Details
95. MligTC455_49378 0.347 Mlig455_044613 SLC2A12; Slc2a12; Tret1-2 Sugar (and other) transporter Details
96. MligTC455_01742 0.347 Mlig455_064066 Details
97. MligTC455_23465 0.346 Mlig455_063585 Details
98. MligTC455_37993 0.345 Mlig455_014151 Wax ester synthase-like Acyl-CoA acyltransferase domain Details
99. MligTC455_30835 0.345 Mlig455_058095 HDAC11; Hdac11 Histone deacetylase domain Details
100. MligTC455_23099 0.342 Mlig455_048061 Details
101. MligTC455_26641 0.341 Mlig455_025147 PLXNB1; PlexA; Plxna4 Plexin cytoplasmic RasGAP domain Details
102. MligTC455_20229 0.338 Mlig455_025947 BLM; Blm DEAD/DEAH box helicase Details
103. MligTC455_10700 0.336 Mlig455_006317 Details
104. MligTC455_37193 0.336 Mlig455_008152 GRIN3B; Grin3a; Nmdar2 Ligand-gated ion channel Details
105. MligTC455_09156 0.336 Mlig455_017160 Globin Details
106. MligTC455_46229 0.336 Mlig455_022523
Mlig455_022563		 Details
107. MligTC455_18976 0.336 Mlig455_035823 Details
108. MligTC455_08861 0.336 Mlig455_041154 Amiloride-sensitive sodium channel Details
109. MligTC455_09157 0.336 Mlig455_048111 Globin Details
110. MligTC455_14928 0.333 Mlig455_002569 CG3328; MYRF; Myrf NDT80 / PhoG like DNA-binding family Details (TF)
111. MligTC455_14929 0.333 Mlig455_057903 CG3328; MYRF; Myrf NDT80 / PhoG like DNA-binding family Details (TF)
112. MligTC455_33392 0.33 Mlig455_020352 Details
113. MligTC455_32122 0.33 Mlig455_046207 Uroporphyrinogen-III synthase HemD Details
114. MligTC455_38346 0.33 Mlig455_055515 Details
115. MligTC455_38347 0.33 Mlig455_055528 Details
116. MligTC455_48313 0.327 Mlig455_007201 CG4324; SVOP; Svop Sugar (and other) transporter Details
117. MligTC455_14090 0.327 Mlig455_013210
Mlig455_013233		 Details
118. MligTC455_25245 0.327 Mlig455_006162
Mlig455_029139
Mlig455_054138
Mlig455_065758
Mlig455_068467
Mlig455_071050		 SPH93 Domain of unknown function (DUF4834);
Repeat of unknown function (DUF1502);
Transcription factor IIA, alpha/beta subunit		 Details
119. MligTC455_43037 0.326 Mlig455_056906
Mlig455_056938		 HUS1; Hus1; Hus1-like Hus1-like protein Details
120. MligTC455_43392 0.324 Mlig455_055609 Armc6; CG5721 Details
121. MligTC455_32066 0.322 Mlig455_048493 MFS18; SLC17A9; Slc17a9 Major Facilitator Superfamily Details
122. MligTC455_40623 0.322 Mlig455_053686
Mlig455_064879		 Histone H1-like nucleoprotein HC2 Details
123. MligTC455_06788 0.321 Mlig455_003273
Mlig455_014182
Mlig455_037567
Mlig455_046981		 Details
124. MligTC455_47371 0.321 Mlig455_051289 PIPOX; Pipox FAD dependent oxidoreductase Details
125. MligTC455_44820 0.32 Mlig455_002329 Details
126. MligTC455_29210 0.317 Mlig455_038508 ANK1; Ank2; Ankrd28 Ankyrin repeats (3 copies) Details
127. MligTC455_50696 0.317 Mlig455_055962 Grsf1; HNRNPF; glo RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) Details
128. MligTC455_43391 0.316 Mlig455_002989 Armc6; CG5721 Details
129. MligTC455_38345 0.315 Mlig455_044919 Details
130. MligTC455_41284 0.314 Mlig455_056317 Details
131. MligTC455_20228 0.311 Mlig455_025853
Mlig455_025915		 BLM; Blm DEAD/DEAH box helicase Details
132. MligTC455_02591 0.31 Mlig455_027318 MX1; Mx2 Dynamin central region Details
133. MligTC455_50610 0.31 Mlig455_037294
Mlig455_037333		 KIF12; Kif12; Klp54D Kinesin motor domain Details
134. MligTC455_18641 0.309 Mlig455_002462 MFSD13A; Mfsd13a MFS/sugar transport protein Details
135. MligTC455_38710 0.309 Mlig455_007324 THAP12; Thap12 Domain of unknown function (DUF4371) Details (TF)
136. MligTC455_22384 0.309 Mlig455_010681 Leucine Rich repeat Details
137. MligTC455_30196 0.309 Mlig455_037120 CBWD2; Cbwd1 CobW/HypB/UreG, nucleotide-binding domain Details
138. MligTC455_28654 0.309 Mlig455_035618
Mlig455_048294		 RNF213; Rnf213 ATPase family associated with various cellular activities (AAA) Details
139. MligTC455_14352 0.309 Mlig455_057678 Details
140. MligTC455_50358 0.309 Mlig455_063419 Thumpd2 Details
141. MligTC455_18543 0.308 Mlig455_041787 FADS2; Fads3 Fatty acid desaturase Details
142. MligTC455_34538 0.306 Mlig455_037040
Mlig455_065619		 NYNRIN Immunoglobulin domain;
Integrase zinc binding domain		 Details
143. MligTC455_48636 0.305 Mlig455_011518
Mlig455_011596		 ANK2; Ankrd6; mask Ankyrin repeats (3 copies) Details
144. MligTC455_22968 0.305 Mlig455_025411 NCK2; Nck1; dock SH3 domain Details
145. MligTC455_54077 0.305 Mlig455_032159
Mlig455_032165		 Cubn; TLL2; tok CUB domain Details
146. MligTC455_46367 0.303 Mlig455_066511 Details
147. MligTC455_23362 0.302 Mlig455_010012 Details
148. MligTC455_41283 0.302 Mlig455_056291 Details
149. MligTC455_50626 0.302 Mlig455_039925
Mlig455_041197
Mlig455_070097		 ANKRD53; Ankrd53; f Ankyrin repeats (many copies) Details
150. MligTC455_36369 0.301 Mlig455_001046 Details
151. MligTC455_13718 0.301 Mlig455_010908 Details
152. MligTC455_00399 0.301 Mlig455_011854 Details
153. MligTC455_53775 0.301 Mlig455_012089 Details
154. MligTC455_23844 0.301 Mlig455_012645 ANKS1A; Anks1; CG4393 Phosphotyrosine interaction domain (PTB/PID) Details
155. MligTC455_23848 0.301 Mlig455_012656 ANKS1A; Anks1; CG4393 Phosphotyrosine interaction domain (PTB/PID) Details
156. MligTC455_35654 0.301 Mlig455_012933 Atg18a; WIPI2; Wipi2 Core histone H2A/H2B/H3/H4 Details
157. MligTC455_12037 0.301 Mlig455_016647
Mlig455_016711		 DYNLL1; Dynll1; ctp Dynein light chain type 1 Details
158. MligTC455_49771 0.301 Mlig455_018423 Details
159. MligTC455_04266 0.301 Mlig455_019932 Zinc finger, C3HC4 type (RING finger) Details
160. MligTC455_04267 0.301 Mlig455_020005 Inhibitor of Apoptosis domain Details
161. MligTC455_04268 0.301 Mlig455_020034 Tetraspanin family Details
162. MligTC455_08244 0.301 Mlig455_022615 VAV1; Vav1 RhoGEF domain Details
163. MligTC455_27506 0.301 Mlig455_023007 TNKS2; Tnks; Tnks2 Ankyrin repeats (3 copies) Details
164. MligTC455_13156 0.301 Mlig455_023459 Details
165. MligTC455_00086 0.301 Mlig455_024697 PT repeat Details
166. MligTC455_55635 0.301 Mlig455_025139 PRR9 Details
167. MligTC455_00778 0.301 Mlig455_027260 Details
168. MligTC455_43851 0.301 Mlig455_031738 CG8483; PI16; Pi16 Cysteine-rich secretory protein family Details
169. MligTC455_22364 0.301 Mlig455_034675 Details
170. MligTC455_27328 0.301 Mlig455_029735
Mlig455_034892		 CG1628; SLC25A12; Slc25a12 Mitochondrial carrier protein Details
171. MligTC455_52288 0.301 Mlig455_035126 Details
172. MligTC455_04387 0.301 Mlig455_036561 Thumpd2 Cysteine rich repeat Details
173. MligTC455_23865 0.301 Mlig455_036682 Details
174. MligTC455_25163 0.301 Mlig455_036820 Details
175. MligTC455_15011 0.301 Mlig455_037518 Details
176. MligTC455_29534 0.301 Mlig455_039000 Details
177. MligTC455_18028 0.301 Mlig455_039411 Details
178. MligTC455_12671 0.301 Mlig455_038144
Mlig455_039808		 Details
179. MligTC455_55601 0.301 Mlig455_043378 CG42322; SLC35F4; Slc35f4 Details
180. MligTC455_13702 0.301 Mlig455_044274 Details
181. MligTC455_12032 0.301 Mlig455_044477 CG5890; KCNIP1; Kcnip1 EF hand Details
182. MligTC455_31743 0.301 Mlig455_046952 Details
183. MligTC455_53531 0.301 Mlig455_047914 TBX2; Tbx2; bi T-box Details (TF)
184. MligTC455_56475 0.301 Mlig455_048650 Details
185. MligTC455_56721 0.301 Mlig455_049301 PRR9 Details
186. MligTC455_53926 0.301 Mlig455_049597 Details
187. MligTC455_36084 0.301 Mlig455_050285 Transforming growth factor beta like domain Details
188. MligTC455_02739 0.301 Mlig455_050666 Reverse transcriptase (RNA-dependent DNA polymerase) Details
189. MligTC455_06713 0.301 Mlig455_053256 Details
190. MligTC455_36566 0.301 Mlig455_053263 Details
191. MligTC455_47129 0.301 Mlig455_054855 Syntaxin 6, N-terminal Details
192. MligTC455_00776 0.301 Mlig455_056451 Details
193. MligTC455_04238 0.301 Mlig455_057696 5-HT1A; HTR1B; Htr1b 7 transmembrane receptor (rhodopsin family) Details
194. MligTC455_36370 0.301 Mlig455_059466 Details
195. MligTC455_36381 0.301 Mlig455_059467 Details
196. MligTC455_07621 0.301 Mlig455_060789
Mlig455_060818		 Domain of unknown function (DUF3504) Details
197. MligTC455_29762 0.301 Mlig455_061431 BEST1; Best1 Bestrophin, RFP-TM, chloride channel Details
198. MligTC455_12886 0.301 Mlig455_062770 Details
199. MligTC455_02357 0.301 Mlig455_063667 Details
200. MligTC455_36567 0.301 Mlig455_065030 Details
201. MligTC455_06759 0.301 Mlig455_066248 PT repeat Details
202. MligTC455_41948 0.301 Mlig455_066265 PT repeat Details
203. MligTC455_18011 0.301 Mlig455_066318 CG42322; SLC35F4; Slc35f4 Details
204. MligTC455_21850 0.301 Mlig455_068061 Details
205. MligTC455_18910 0.301 Mlig455_069210 Details
206. MligTC455_53074 0.301 Mlig455_069508 CASK; Cask Protein kinase domain Details
207. MligTC455_52864 0.301 Mlig455_070404 Details
208. MligTC455_20152 0.3 Mlig455_022732 Details

Similar genes by variation

Nr. Mlig455 TC Pearson corr. Mlig455 genes Homologs Pfam Full annotation
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